High Resolution Light Microscopy of Live Cells
نویسندگان
چکیده
منابع مشابه
High-Resolution Light-Field Microscopy
By combining a high-resolution image from a standard camera with a low-resolution wavefront measurement from a Shack-Hartmann sensor, we numerically reconstruct a highresolution light field. We experimentally demonstrate the method with a commercially available microscope. ©2013 Optical Society of America OCIS codes: (110.0180) Microscopy; (110.1758) Computational imaging; (100.6640) Superresol...
متن کاملLight-induced cell damage in live-cell super-resolution microscopy
Super-resolution microscopy can unravel previously hidden details of cellular structures but requires high irradiation intensities to use the limited photon budget efficiently. Such high photon densities are likely to induce cellular damage in live-cell experiments. We applied single-molecule localization microscopy conditions and tested the influence of irradiation intensity, illumination-mode...
متن کاملHigh-Resolution Imaging of Mitochondria in Live Cells
Cardiac mitochondria are complex highly organized cellular organelles, which play central roles not only in energy homeostasis but also in various biosynthetic, signaling, and cell death pathways. Moreover, mitochondria are highly dynamic organelles that continuously divide and fuse as well as move within the cell. Therefore, it is not surprising that multiple methodological approaches have bee...
متن کاملHigh-Resolution Intravital Microscopy
Cellular communication constitutes a fundamental mechanism of life, for instance by permitting transfer of information through synapses in the nervous system and by leading to activation of cells during the course of immune responses. Monitoring cell-cell interactions within living adult organisms is crucial in order to draw conclusions on their behavior with respect to the fate of cells, tissu...
متن کاملHigh Resolution Scanning Electron Microscopy of Cells Using Dielectrophoresis
Ultrastructural analysis of cells can reveal valuable information about their morphological, physiological, and biochemical characteristics. Scanning electron microscopy (SEM) has been widely used to provide high-resolution images from the surface of biological samples. However, samples need to be dehydrated and coated with conductive materials for SEM imaging. Besides, immobilizing non-adheren...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy Today
سال: 2005
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500051609